Applications of NaveniFlex proximity ligation technology

With NaveniFlex you can detect protein-protein interactions, post-translational modifications, for example phosphorylation, localize endogenous proteins with higher specificty and study receptor activation with the possibility of relative quantification. All NaveniFlex products are based on secondary detection by Navenibodies (carefully selected seconday antibodies conjugated to proprietary oligo arms). The Navenibodies enable a highly sensitive and specific readout of the selected target.

Choose kit by application

Application NaveniFlex MR NaveniFlex GR NaveniFlex MM NaveniFlex RR
Protein interactions
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X
Protein modifications
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X
Protein expression
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X
X
X

 

Biomarker targets

NaveniFlex MR

Protein interaction: Chromatin interactions (histone H3) with the nuclear lamina (lamin-B1).

Cell line: BT474 (breast cancer cells)

Detection fluorophore: TEX615

Protein interaction of beta-catenin and e-cadherin

Tissue Human skin tissue

Detection fluorophore: ATTO488

Protein interaction of beta-catenin and e-cadherin

Cells MCF7 cells

Detection fluorophore: Atto647N

Protein detection HER2, protein detection

Cells  BT474 (breast cancer cells)

Detection fluorophore: TEX615

NaveniFlex MM

Protein detection CA125

Tissue: CA-125, 5 + Control Slide, Sigma Aldrich

Detection fluorophore: TEX615

Protein detection Histone H3

Cells: BT474 (breast cancer cells)

Detection fluorophore: TEX615

Protein detection Histone H3

Tissue: Human skin

Detection fluorophore: TEX615

NaveniFlex RR

Protein detection PDL1

Cells: HDLM-2, SignalSlide® PD-L1 IHC Controls 13747

Detection fluorophore: TEX615

Protein detection: Lamin-B1

Cells: BT474 (breast cancer cells)

Detection fluorophore: TEX615