NaveniFlex in Autophagy publication
We’re excited to see that our Naveni® technology has been referenced in a fantastic publication describing novel insights into the process of lysosomal degradation of mitochondria (mitophagy) (Godtliebsen et al. 2023, Autophagy).
The authors used ingenious double tagging of mitochondria, where the green signal was quenched at low pH, whereas red was acid-stable. To induce a metabolic shift to oxidative phosphorylation, they adapted rat cardiac myoblasts to galactose in glucose-free medium and observed that lead to lysosomal engulfment and fragmentation of mitochondria seen as red-only (acidic) structures.
Navinci’s NaveniFlex™ MR kit was used to visualize the interaction between the matrix protein PDHA1 and the autophagy marker MAP1LC3B on mitochondria and on red-only dots. It revealed that the interaction was only present in mitochondria but not on red-only dots, with no difference between Gal-adapted vs control cells. This suggests that autophagy is ongoing in mitochondria before acidification, but also points towards MAP1LC3B not being the main mediator of enhanced mitochondrial degradation in conditions of oxidative phosphorylation. The implied redundancy in the degradation mechanisms only goes to underscore the crucial role of mitophagy.