Description
In in situ proximity ligation assay (isPLA), successful detection depends on efficient antibody access to target epitopes. During fixation – particularly with formaldehyde or FFPE processing – epitopes can become cross-linked or masked, limiting probe binding and reducing signal output.
Navinci’s Antigen Retrieval Buffer is designed to effectively unmask these epitopes, restoring accessibility and enabling optimal binding of isPLA probes. The result is improved signal generation, enhanced sensitivity, and more consistent assay performance.
Developed and validated within Navinci workflows, this high pH Tris-EDTA buffer delivers robust, reproducible performance and is optimized for Heat-Induced Epitope Retrieval (HIER).
Read our technology note about the Navinci Antigen Retrieval Buffer here.
Recommended use:
- Optimized for Navinci manual targeted kits
- Suitable for Navinci manual flexible kits – depending on selected primary antibodies
For Navinci kits, using this buffer ensures a fully validated workflow – reducing troubleshooting and giving you confidence in your results.
Documents
| Type | Version | Date | Download |
| Instructions for Use | 1.0 | 29-04-26 | |
| Safety Data Sheet | 1.0 | 29-04-26 | |
| Technology note | 1.0 | 29-04-26 |