The Naveni PD1/PD-L1 AP is an in situ proximity ligation assay kit for chromogenic detection of the interaction of PD1 and PD-L1. This is the first commercial assay for detecting the interaction in situ, the kit gives a high signal-to-noise ratio and has an out-standing specificity. The kit has been validated on FFPE human tissue samples. For research use only. Not for use in diagnostic procedures.
The kit is based on our Naveni® Proximity Ligation Technology, with two Navenibodies conjugated to proprietary oligo arms. The technology ensures specific and sensitive detection. One Navenibody binds the PD1 protein, and the other Navenibody binds PD-L1. Probes in close proximity will give a strong and distinct signal, creating a specific and sensitive detection method for the interaction between PD1 and PD-L1.
Product code: PPI.PDL1.AP.100
Included in the kit:
- Navenibody targeting human PD1 protein based on clone EH33 CST
- Navenibody targeting human PD-L1 protein based on clone SP142 Abcam RabMAb®
- AP substrate, red precipitating reaction product
- Buffers for blocking and dilutions and all detection reagents
- The Navenibody solution has a working volume of 4000µl (sufficient for approx. 100 reactions)
Not included in the kit:
- VectaMount® Express Mounting Medium (H-5700-60) from Vector Laboratories (required for optimal signal intensity).
- Isopropanol 99,5 %.
- Endogenous quenching solution.
- TBS and TBS-T – Tris-buffered saline and Tris-buffered saline supplemented with 0,05% Tween, respectively
Product is also available with HRP substrate.
Find better prognostic biomarkers
PD1 PD-L1 signaling has with immune checkpoint inhibitors proven to be significant in cancer progression and treatment. Despite the recent success of immune checkpoint inhibitors, many patients do not benefit from these therapies, and predictive biomarkers improving patient stratification are needed.PD-L1 IHC is commonly used as a biomarker, but the correlation between PD-L1 expression levels and PD1/PD-L1 interaction is not always linear.
Proteins perform a wide range of functions and are the closest way to understand biology. Proteins are also key targets in both health and disease. When proteins carry out their function, they interact with other proteins or molecules. To understand biological pathways and mode of action, we need to get a deeper insight into protein functions and how the protein interacts. Detecting the interactions has until now been either complicated, unspecific, or insensitive. The Naveni® Proximity Ligation Technology enables visualization of protein-protein interaction using one primary antibody for each target protein, without the need to disrupt the tissue microenvironment.
Keywords: Proximity Ligation Assay, protein protein interaction (PPI), in situ, bright-field, chromogenic, immunohistochemistry, western blot, validated antibodies, Immuno-oncology, immunotherapy, patient stratification, biomarker, drug-target engagement, tissue microenvironment, single-cell resolution, BRET, FRET, Co-IP,