29.500 kr

Visualize the interaction of PD1/PD-L1 in situ using BOND RX/RXm Fully Automated Research Stainer.

Contact us
Category: SKU: NAB.MR.030.H


The Naveni® PD1/PD-L1 BOND RX HRP is an in situ proximity ligation assay kit for the detection of the interaction of PD1 and PD-L1 using BOND RX/RXm Fully Automated Research Stainer. The kit has been validated on FFPE human tissue samples. Not recommend for mouse tissue. For research use only. Not for use in diagnostic procedures.

The kit is based on the Naveni® in situ proximity ligation technology with automated workflow. It uses two Navenibodies linked to proprietary oligo arms. The primary antibodies included in the kit bind to PD1 and PD-L1 proteins, while Navenibodies then bind to their respective primary antibodies. When Navenibodies are in close proximity, a strong and distinct signal is generated, offering a specific and sensitive detection method for the interaction between PD1 and PD-L1. Read more about the automated technology here.

Product code: NA.PPI01.030.H


Included in the Naveni® PD1/PD-L1 BOND RX HRP kit:

  • Navenibody targeting human PD1 protein based on clone EH33 CST
  • Navenibody targeting human PD-L1 protein based on clone SP142 Abcam RabMAb®
  • Buffers for blocking and dilutions and detection reagents for the PD1/PD-L1 interaction signal
  • Reagents sufficient for 30 FFPE tissue slides, including dead volumes


Not included in the kit:

Leica Biosystems for BOND RX/RXm with standard accessories

Catalog nr Count Description
OPT9049 1 BOND titration kit
DS9263 or
BOND Intense R
BOND Polymer Refine
AR9590 1 BOND Wash solution 10x concentrate
AR9222 1 BOND Dewax solution
AR9961 1 BOND Epitope retrival solution 1
AR9640 1 BOND Epitope retrival solution 2


Other reagents required
Mounting medium




Find better prognostic biomarkers

PD1 PD-L1 signaling has with immune checkpoint inhibitors proven to be significant in cancer progression and treatment. Despite the recent success of immune checkpoint inhibitors, many patients do not benefit from these therapies, and predictive biomarkers improving patient stratification are needed. PD-L1 IHC is commonly used as a biomarker, but the correlation between PD-L1 expression levels and PD1/PD-L1 interaction is not always linear.

Naveni® PPI

Proteins perform a wide range of functions and are the closest way to understand biology. Proteins are also key targets in both health and disease. When proteins carry out their function, they interact with other proteins or molecules. To understand biological pathways and mode of action, we need to get a deeper insight into protein functions and how the protein interacts. Detecting the interactions has until now been either complicated, unspecific, or insensitive. The Naveni® in situ proximity ligation technology enables visualization of protein-protein interaction using one primary antibody for each target protein, without the need to disrupt the tissue microenvironment.

Keywords: Proximity Ligation Assay, protein protein interaction (PPI), in situ, bright-field, chromogenic, immunohistochemistry, western blot, validated antibodies, Immuno-oncology, immunotherapy, patient stratification, biomarker, drug-target engagement, tissue microenvironment, single-cell resolution, BRET, FRET, Co-IP.

Additional information



Samples type

Cells, Tissue






Automated with BOND RX/RXm

Contact us