Description
Naveni® Plex is the first multiplex in situ proximity ligation assay, with a fluorescent readout, enabling the simultaneous detection of 3 separate targets or protein-protein interactions, with enhanced specificity and sensitivity. The kit has been validated on FFPE human tissue samples.
The kit is based on the Naveni® in situ proximity ligation technology, with target specific primary Naveinbodies conjugated to proprietary oligo arms. Each target has a unique pair of Navenibodies, either directed against two separate proteins, for detecting interactions, or two separate epitopes on the same protein, for specific single protein detection. When the Navenibodies have bound to their respective targets and are in close proximity, a strong and distinct signal is generated. Each target uses separate fluorophores to make simultaneous detection possible. In this kit, we have expanded on our Naveni® technology to detect multiple targets simultaneously for the first time – find out more about the Naveni® Plex technology and workflow here.
Not recommended for mouse tissue. For research use only. Not for diagnostic procedures.
Product code: 60000
Included in the Naveni® Plex kit:
- Naveni Plex Navenibodies A PD1/ PD-L1 interaction
- Naveni Plex Navenibodies B CD3 protein
- Naveni Plex Navenibodies C CD8/ MHC-I interaction
- Buffers for blocking, dilutions and detection reagent mix (ABC)
Required Materials and Reagents to run Naveni® Plex:
These materials are not included in the kit and must be purchased separately.
| Reagent | Vendor, Cat# |
|---|---|
| Xylene | |
| Ethanol – 70, 95 and 99.5% | |
| EDTA antigen retrieval buffer | Sigma-Aldrich, E1161-100ML |
| Tris Buffered Saline with 0.05% Tween 20 (TBS-T) | |
| Tris Buffered Saline (TBS) | |
| Phosphate Buffered Saline (PBS) | |
| Fluoroshield mounting medium | Thermo Fisher Scientific, F6182 |
| DAPI (Diluted in PBS to 1µg/ mL) | Thermo Fisher Scientific, 62248 |
Documents
- Instructions For Use Naveni® Plex (PDF)
- Naveni® Plex Product Sheet (PDF)
- Safety Data Sheet Naveni® Plex (PDF)
- Frequently Asked Questions
Understand immune evasion and mechanisms
Estimate the relative levels of T-cell infiltration (CD3 and CD8), cytotoxic engagement (CD8/MHC-I), and immune inhibition (PD1/PD-L1) to investigate how immune evasion correlates with T-cell activation and gain insights into tumors blocking immune attacks.