Naveni pTyr EGFR

The Naveni pTyr EGFR target specific assay detects phosphorylated EGFR with high specificity and sensitivity using our patented Naveni™ probe technology. The kit includes primary Navenibodies against phosphorylated EGFR and all other reagents needed to run the assay.

Category: SKU: NPT.EGFR

Description

The Naveni pTyr EGFR is an in situ proximity ligation assay kit for detecting phosphorylated epidermal growth factor receptor (EGFR/HER1), with very high sensitivity and specificity. The proximity ligation signal has a high signal-to-noise ratio and is easily quantifiable, contrary to traditional immunofluorescence.

The Navenibodies binds two epitopes on the phosphorylated EGFR. Targeting two epitopes increases the specificity of the signal significantly. One Navenibody targets the protein close to the cytoplasmic C-terminal, and the other Navenibody interacts with phosphorylated tyrosine residues. Probes in close proximity will give rise to an strong and distinct signal, creating a specific and sensitive detection method for phosphorylated EGFR.

Price available upon request

 

The Naveni pTyr EGFR assay kit contains the following reagents

The kit consists of two boxes that are stored separately.

Box 1 contains Blocking buffer, Navenibody diluent. Stored at +4 °C to +8 °C.

Box 2 contains two Navenibodies, one targeting cytoplasmic human EGFR protein, the other a broad range of tyrosine-phosphorylated proteins and peptides, as well as detection reagents. Stored at -15 °C to -25 °C.

The reagents, that upon dilution, gives 4 ml of working solution, which allows 100 tests á 40 µL. The reaction volume of 40 µL is sufficient to cover 1 cm2 sample on a slide. The volume must be adjusted according to the reaction area and the number of samples.

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