Description
The Naveni® pY HER2 is an in situ proximity ligation assay kit for fluorescence detection of phosphorylated HER2. The kit gives a high signal-to-noise ratio and is easily quantifiable, contrary to traditional immunofluorescence. The kit is based on our Naveni® in situ proximity ligation technology, with two Navenibodies conjugated to proprietary oligo arms and ensures specific and sensitive detection.
The Navenibodies in the Naveni® pY HER2 kits targets two epitopes on the phosphorylated HER2. One Navenibody targets the protein close to the cytoplasmic C-terminal, and the other Navenibody interacts with phosphorylated tyrosine residues. Probes in close proximity will give rise to a strong and distinct signal, creating a specific and sensitive detection method for phosphorylated HER2. The kit is optimized for cell samples, and everything is included.
Product code: NPT.HER2
Included in the kit:
- Navenibody targeting cytoplasmic human HER2 protein
- Navenibody targeting phosphorylated tyrosine residues
- Detection fluorophore TEX615
- Buffers for blocking and dilutions, and all detection reagents
- The Navenibody solution has a working volume of 4000µl (sufficient for approx. 100 reactions)
User instructions
Phosphorylated HER2, a well-known cancer marker
Human epidermal growth factor receptor 2 (HER2), also known as HER2/neu, erbB-2, and proto-oncogene Neu plays a significant role in the pathogenesis of human breast cancer. HER2 has become an important biomarker for breast cancer therapy. Over-expression of HER2 is also associated with other cancer forms, like gastric, esophageal, endometrial, lung cancer, etc. Despite the vital role in tumorigenesis, the role of activation and phosphorylation in situ has not been well described. With Naveni® pY HER2 you can maximize the information from your cell samples and quantify phosphorylation of HER2 in situ with sensitivity and specificity.
Naveni® PTM
Posttranslational modification (PTM), such as tyrosine phosphorylation (pTyr, pY) of proteins, is essential for several cellular functions and pathophysiological processes. The Naveni® PTM product line includes kits for detecting tyrosine phosphorylation of RTKs. Western blot has traditionally been used to study phosphorylation of proteins, but specific tools for quantifying phosphorylation of RTKs in situ have been requested for a long time. The Naveni® PTM kits now make it possible to detect and quantify low-abundant phosphorylated RTKs in situ with single-cell resolution.
Keywords: Proximity Ligation Assay, Phosphorylation, phosphorylated proteins, posttranslational modification (PTM), in situ, receptor tyrosine kinases (RTKs), fluorescence, western blot, validated antibodies