The Naveni® pY EGFR is an in situ proximity ligation assay kit for fluorescence detection of phosphorylated EGFR. The kit gives a high signal-to-noise ratio and is easily quantifiable, contrary to traditional immunofluorescence. The kit is based on our Naveni® in situ proximity ligation technology, with two Navenibodies conjugated to proprietary oligo arms. The technology ensures specific and sensitive detection.
The Navenibodies in the Naveni® pY EGFR kits targets two epitopes on the phosphorylated EGFR. One Navenibody targets the protein close to the cytoplasmic C-terminal, and the other Navenibody interacts with phosphorylated tyrosine residues. Probes in close proximity will give rise to a strong and distinct signal, creating a specific and sensitive detection method for phosphorylated EGFR. The kit is optimized for cell samples, and everything is included.
Product code: NPT.EGFR
Included in the kit:
- Navenibody targeting cytoplasmic human EGFR protein
- Navenibody targeting phosphorylated tyrosine residues
- Detection fluorophore TEX615
- Buffers for blocking and dilutions, and all detection reagents
- The Navenibody solution has a working volume of 4000µl (sufficient for approx. 100 reactions)
Accurately quantify the level of phosphorylated EGFR in your cells
Epidermal growth factor receptor (EGFR) is like the other RTKs essential in regulating cell proliferation, differentiation, migration, and survival. Dysregulation of EGFR underlies several diseases but is mainly associated with different cancers. Naveni® pY EGFR is a new tool for the in situ visualization and quantification of EGFR phosphorylation in cell samples, making it possible to get accurate information about the level of phosphorylation.
Posttranslational modification (PTM), such as tyrosine phosphorylation (pTyr, pY) of proteins, is essential for several cellular functions and pathophysiological processes. The Naveni® PTM product line includes kits for detecting tyrosine phosphorylation of RTKs. Western blot has traditionally been used to study phosphorylation of proteins, but specific tools for quantifying phosphorylation of RTKs in situ have been requested for a long time. The Naveni® PTM kits now make it possible to detect and quantify low-abundant phosphorylated RTKs in situ with single-cell resolution.
Keywords: Proximity Ligation Assay, Phosphorylation, phosphorylated proteins, posttranslational modification (PTM), in situ, receptor tyrosine kinases (RTKs), fluorescence, western blot, validated antibodies