The Naveni® pY MET is an in situ proximity ligation assay kit for fluorescence detection of phosphorylated Met. The kit gives a high signal-to-noise ratio and is easily quantifiable, contrary to traditional immunofluorescence. The kit is based on our Naveni® in situ proximity ligation technology, with two Navenibodies conjugated to proprietary oligo arms. The technology ensures specific and sensitive detection; learn more about our Naveni® in situ proximity ligation technology.
The Navenibodies in the Naveni® pY MET kits targets two epitopes on the phosphorylated MET. One Navenibody targets the protein close to the cytoplasmic C-terminal, and the other Navenibody interacts with phosphorylated tyrosine residues. Probes in close proximity will give rise to a strong and distinct signal, creating a specific and sensitive detection method for phosphorylated MET. The kit is optimized for cell samples, and everything is included.
Product code: NPT.MET
Included in the kit:
- Navenibody targeting cytoplasmic human MET protein
- Navenibody targeting phosphorylated tyrosine residues
- Detection fluorophore TEX615
- Buffers for blocking and dilutions, and all detection reagents
- The Navenibody solution has a working volume of 4000µl (sufficient for approx. 100 reactions)
Phosphorylated MET – a driver of tumor progression
The receptor tyrosine kinase c-MET (hepatocyte growth factor receptor) activates intracellular signaling and induces cell proliferation, epithelial-to-mesenchymal-transition, and migration. C-MET is hyperactivated in certain tumors and has been reported to drive tumor progression, therapy resistance, and metastatic spread. There is a need for more detailed knowledge about biological mechanisms and regulatory networks for future patient stratification and individualized treatment concepts. With the new Naveni® pY MET assay, you can detect phosphorylated MET with very high sensitivity and specificity.
Posttranslational modification (PTM), such as tyrosine phosphorylation (pTyr, pY) of proteins, is essential for several cellular functions and pathophysiological processes. The Naveni® PTM product line includes kits for detecting tyrosine phosphorylation of RTKs. Western blot has traditionally been used to study phosphorylation of proteins, but specific tools for quantifying phosphorylation of RTKs in situ have been requested for a long time. The Naveni® PTM kits now make it possible to detect and quantify low-abundant phosphorylated RTKs in situ with single-cell resolution.
Keywords: Proximity Ligation Assay, Phosphorylation, phosphorylated proteins, posttranslational modification (PTM), in situ, receptor tyrosine kinases (RTKs), fluorescence, western blot, validated antibodies