The Naveni TriFlex Cell MR is an in situ kit, to be used with the user’s primary antibodies of choice (raised in mouse and rabbit). The kit enables fluorescent staining of protein-protein interactions, concurrently visualizing the two proteins in their free state. Compatible with traditional immunostaining equipment, the kit uses Navenibody detection to produce the amplified signals facilitating immediate quantification of the readout, three fluorophores are included requiring the filters FITC, Cy3 and Cy5.
The kit is based on our Naveni® Proximity Ligation Technology, with TriFlex Navenibodies conjugated to proprietary oligo arms. The technology ensures specific and sensitive detection (learn more about Naveni TriFlex here). The kit has been validated on cell samples.
Product code: TF.MR.100
Included in the kit:
- Anti-mouse Navenibody
- Anti-rabbit Navenibody
- Three detection fluorophores requiring filter sets for FITC (rabbit antibody signal), Cy3 (mouse antibody signal), Cy5 (proximity signal).
- Buffers for blocking and dilutions, and all detection reagents
- The Navenibody solution has a working volume of 4000µl (sufficient for approx. 100 reactions)
Not included in the kit:
- Primary antibodies; use a mouse and a rabbit antibody. If you are unsure how to choose a primary antibody for your protein of interest, please contact us for advice and support.