Cellular processes can only be understood as the dynamic interplay of proteins. This means that we need accurate techniques to monitor interactions of endogenous proteins directly in individual cells and tissues, to reveal the cellular and molecular architecture and its responses to disturbances.
Proximity Ligation Assay is a cutting-edge technology that has revolutionized the way researchers study protein interactions and protein interplay.
In traditional methods, detecting protein interactions and cellular localization involved laborious and time-consuming procedures such as immunoprecipitation, BRET, and cross-linking methods. The advent of Proximity Ligation Assay has made it possible to perform these experiments in a faster and more efficient manner, with high sensitivity and specificity.