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Integrating in situ proximity ligation assay with multiplexed immunofluorescence imaging

Presented at AACR24 by Tony Ullman, a PhD student in Dr Charlotte Stadler’s lab at KTH-SciLifeLab, is the poster “Integrating in situ Proximity Ligation Assay for PD-1/PD-L1 interaction with Multiplexed Immunofluorescence Imaging in Urothelial Carcinoma”

Integrating in situ proximity ligation assay with multiplexed immunofluorescence imaging

Presented at AACR24 by Tony Ullman, a PhD student in Dr Charlotte Stadler’s lab at KTH-SciLifeLab, is the poster “Integrating in situ Proximity Ligation Assay for PD-1/PD-L1 interaction with Multiplexed Immunofluorescence Imaging in Urothelial Carcinoma”

Charlotte Stadler’s group at SciLife-KTH used the PD1/PDL1 interaction as part of a multiplex IF panel with the aim to deep-phenotype the TME. They succeeded in fully integrating the isPLA on the Leica BOND and Phenocycler platforms. Next, they used the developed protocol to compare responders vs non-responders from a retrospective ICT-treated bladder cancer cohort based on the abundance of detected PD1/PDL1 interactions. In their proof-of-concept experiments, they demonstrated that increased number of interactions correlated with ICT response.​​

Charlotte Stadler’s group at SciLife-KTH used the PD1/PDL1 interaction as part of a multiplex IF panel with the aim to deep-phenotype the TME. They succeeded in fully integrating the isPLA on the Leica BOND and Phenocycler platforms. Next, they used the developed protocol to compare responders vs non-responders from a retrospective ICT-treated bladder cancer cohort based on the abundance of detected PD1/PDL1 interactions. In their proof-of-concept experiments, they demonstrated that increased number of interactions correlated with ICT response.​​

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Integrating in situ proximity ligation assay with multiplexed immunofluorescence imaging

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