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Quantifying proteins, their interactions and modifications, at the molecular level

The Naveni Technology Platform pushes the boundaries of fluorescence-based in situ methodology, enabling researchers to get the maximum information from every analysis by visualizing and quantifying proteins, their interactions and modifications, in situ at the molecular level. An ability to detect even low abundant proteins ensures that proteins can be investigated without the need to overexpress or modify in any way the natural environment of the cell. Data generated contributes to a deeper understanding of the biological mechanisms within diseased or normal tissues, responses to therapeutic drugs or changes to the cellular microenvironment. The Naveni Technology Platform provides clean, highly resolved images, ready for quantification. 

 

The benefits of our technology are:

  • Clear target detection
  • Consistency in staining
  • Reproducibility
  • Possibility to detect extremely low abundant proteins
  • Detection of protein-protein interactions
  • Specific detection of post-translational modifications, also with the use of pan-specific antibodies

Our technology is tailored for academic and industrial researchers working in fields of:

  • Immuno-profiling
  • Signaling profiling
  • Drug testing
  • Treatment validation
  • Biomarker discovery
  • Diagnostics
 

Workflow

Primary antibody incubation

Two primary antibodies bind to their target epitopes, located on a single protein or on two nearby proteins.

Navenibody incubation

Navenibodies (carefully selected secondary antibodies conjugated to proprietary oligo arms) bind their respective primary antibodies. 

Reaction A

An enzymatic reaction activates the oligos conjugated to the Navenibodies. This increases the efficacy of signal generation and improves the signal strength. 

Reaction B

Only the Navenibodies in close proximity will connect, guaranteeing high specificity by reducing nonspecific background staining.

Reaction C

Fluorescent signals are amplified. The high signal strength enables the detection of separate proximity events, allowing for a resolution down to single protein or protein-protein interaction. 

Visualization 

The results can be visualized with a fluorescence microscope.

Field of interest

We want to know more about the field of research you work in, what challenges you encounter, and what kind of protein targets you are interested in. Please get in touch with us and we will be happy to discuss how the Navinci platform can improve your research.

Publications and downloads

Publications

Klaesson A, Grannas K, Ebai T, Heldin J, Koos B, Leino M, Raykova D, Oelrich J, Arngården L, Söderberg
O, Landegren U. Improved efficiency of in situ protein analysis by proximity ligation using UnFold
probes. Sci Rep. 2018 Mar 29;8(1):5400. PMID: 29599435

Zimmerli D. et al. TBX3 acts as tissue-specific component of the Wnt-beta cathenin transcriptional complex. eLife 2020;9:e58123