Correct assessments of protein localization and consistency in generated data are essential for scientific discovery, translational science, and clinical research. Low-quality data are predisposed to biased judgments and faulty outcomes. Navinci Diagnostics develops and distributes kits for studies of the proteome’s spatial organization and function.
The Navinci kits are based on our proprietary Unfolding Proximity Probes technology. It combines antibody-based proteomic technologies with fluorescence readout to create next-generation immunostaining where sensitivity and specificity meet in one assay, enabling high-quality protein detection and analysis in cells and tissues.
The benefits of our technology are:
Our technology is tailored for academic and industrial researchers working in fields of:
Primary antibody incubation
Two primary antibodies bind to their target epitopes, located on a single protein or on two nearby proteins.
To complete the dual binding, Navinci Universal Probes recognize their respective primary antibodies.
The locked probes are activated by an enzymatic step that increases the efficacy of signal generation, thus improving assay sensitivity.
Only the probes that are within close proximity connect, guaranteeing high specificity by reducing nonspecific background staining.
Fluorescent signals are amplified. The high signal strength enables the detection of separate proximity events, allowing for a resolution down to single protein or protein interaction.
Visualization and data processing
The results can be visualized with a fluorescence microscope
Klaesson A, Grannas K, Ebai T, Heldin J, Koos B, Leino M, Raykova D, Oelrich J, Arngården L, Söderberg
O, Landegren U. Improved efficiency of in situ protein analysis by proximity ligation using UnFold
probes. Sci Rep. 2018 Mar 29;8(1):5400. PMID: 29599435