Naveni® kits for BOND RX/RXm
Automated detection of protein-protein interactions and post-translational modifications in situ
Building on the success of our initial Naveni® in situ kits, we are now introducing two products with fluorescence readout: NaveniFlex™ BOND RX Atto647N/Red and Naveni® PD1/PD-L1 BOND RX Atto647N/Red. These products expand the capabilities of in situ proximity ligation assays with automated workflows on the BOND RX/RXm Fully Automated Research Stainer by Leica Biosystems to achieve increased throughput and consistent high-quality results.
Our portfolio now includes both chromogenic and fluorescence readouts, providing researchers with greater flexibility for in situ detection. Based on the innovative Naveni® in situ proximity ligation technology, these kits are designed for seamless integration into your automated research processes, ensuring reliability, sensitivity, and ease of use.
Automated detection of protein-protein interactions and post-translational modifications in situ
Building on the success of our initial Naveni® in situ kits, we are now introducing two products with fluorescence readout: NaveniFlex BOND RX Atto647N/Red and Naveni® PD1/PD-L1 BOND RX Atto647N/Red. These products expand the capabilities of in situ proximity ligation assays with automated workflows on the BOND RX/RXm Fully Automated Research Stainer by Leica Biosystems to achieve increased throughput and consistent high-quality results.
Our portfolio now includes both chromogenic and fluorescence readouts, providing researchers with greater flexibility for in situ detection. Based on the innovative Naveni® in situ proximity ligation technology, these kits are designed for seamless integration into your automated research processes, ensuring reliability, sensitivity, and ease of use.
- Detect protein-protein interactions and post-translational modifications efficiently using the BOND RX/RXm, ensuring high precision and accuracy.
- Visualize specific protein targets like PD1/PD-L1 while preserving intact cell and tissue morphology, providing a holistic understanding of spatial protein relationships.
- Enhance your research efficiency and reproducibility through a seamlessly integrated automated workflow on BOND RX/RXm, optimizing your investigative process and achieving high throughput.
- Save valuable time with the automated workflow, reducing hands-on time and maximizing overall research productivity.
- FFPE optimized, reagents sufficient for 30 FFPE tissue slides.
- Detect protein-protein interactions and post-translational modifications efficiently using the BOND RX/RXm, ensuring high precision and accuracy.
- Visualize specific protein targets like PD1/PD-L1 while preserving intact cell and tissue morphology, providing a holistic understanding of spatial protein relationships.
- Enhance your research efficiency and reproducibility through a seamlessly integrated automated workflow on the Leica BOND RX/RXm, optimizing your investigative process and achieving high throughput.
- Save valuable time with the automated workflow, reducing hands-on time and maximizing overall research productivity.
- FFPE optimized, reagents sufficient for 30 FFPE tissue slides.
Staining of CD8/MHC-I interaction (pink) in human FFPE breast cancer tissue using NaveniFlex BOND RX Atto647N, co-staining using pan-cytokeratin (green), CD3 (yellow), and DAPI (blue). Note: co-staining with immunofluorescence requires custom protocols.
Staining of CD8/MHC-I interaction (pink) in human FFPE breast cancer tissue using NaveniFlex BOND RX Atto647N, co-staining using pan-cytokeratin (green), CD3 (yellow), and DAPI (blue). Note: co-staining with immunofluorescence requires custom protocols.
Precise Visualization of PD1/PD-L1 Interactions using Naveni® PD1/PD-L1 BOND RX
The Naveni® PD1/PD-L1 BOND RX HRP offer a comprehensive solution for studying PD1/PD-L1 interactions within the tissue microenvironment. With precise visualization capabilities, researchers can gain deeper insights into the dynamics of immune checkpoint pathways, facilitating the development of targeted immunotherapies for cancer treatment.
Naveni® PD1/PD-L1 BOND RX HRP was used to detect PD1/PD-L1 interaction in head neck cancer (A, B), Adrenal gland was used as neg biological control (C).
Naveni® PD1/PD-L1 BOND RX Atto647N was used to stain PD1/PD-L1 interaction in head and neck cancer array, tissues as follows: (A) Squamous cell carcinoma Malignant Tumor Stage I, (B) Squamous cell carcinoma Malignant Tumor Stage III, (C) Undifferentiated carcinoma Malignant Tumor Stage II, (D) Phenochromocytoma (tissue marker) adrenal gland (biological negative control).
Precise Visualization of PD1/PD-L1 Interactions using Naveni® PD1/PD-L1 BOND RX
The Naveni® PD1/PD-L1 BOND RX HRP and the Naveni® PD1/PD-L1 BOND RX Atto647N/Red offer a comprehensive solution for studying PD1/PD-L1 interactions within the tumor microenvironment. With precise visualization capabilities, researchers can gain deeper insights into the dynamics of immune checkpoint pathways, facilitating the development of targeted immunotherapies for cancer treatment.
Naveni® PD1/PD-L1 BOND RX HRP was used to detect PD1/PD-L1 interaction in head neck cancer (A, B), Adrenal gland was used as neg biological control (C).
Naveni® PD1/PD-L1 BOND RX Atto647N was used to stain PD1/PD-L1 interaction in head and neck cancer array, tissues as follows: (A) Squamous cell carcinoma Malignant Tumor Stage I, (B) Squamous cell carcinoma Malignant Tumor Stage III, (C) Undifferentiated carcinoma Malignant Tumor Stage II, (D) Phenochromocytoma (tissue marker) adrenal gland (biological negative control).
Investigating Protein-Protein Interactions
Naveni® kits for BOND RX/RXm Fully Automated Research Stainer enable researchers to explore complex protein-protein interactions with high precision and accuracy using their own primary antibodies. By visualizing these interactions within the spatial context of cells and tissues, researchers can unravel intricate signaling networks and uncover novel therapeutic targets for various diseases.
An example is the interactions between CD8 and MHC-I. Understanding these interactions is vital for elucidating the role of cytotoxic T cells in immune surveillance and their involvement in diseases such as cancer and autoimmune disorders.
Staining of CD8/MHCI interaction in breast cancer using NaveniBright™ BOND RX HRP (A, B positive signals, C negative control)
Investigating Protein-Protein Interactions
Naveni® kits for BOND RX/RXm Fully Automated Research Stainer enable researchers to explore complex protein-protein interactions with high precision and accuracy using their own primary antibodies. By visualizing these interactions within the spatial context of cells and tissues, researchers can unravel intricate signaling networks and uncover novel therapeutic targets for various diseases.
An example is the interactions between CD8 and MHC-I. Understanding these interactions is vital for elucidating the role of cytotoxic T cells in immune surveillance and their involvement in diseases such as cancer and autoimmune disorders.
Staining of CD8/MHCI interaction in breast cancer using NaveniBright BOND RX HRP (A, B positive signals, C negative control)
Understanding Protein Function and Signaling Pathways
With Naveni® kits for BOND RX/RXm, researchers can investigate protein function and signaling events within the tumor microenvironment, shedding light on key biological processes and uncovering new avenues for therapeutic intervention. An example is the dynamic interplay between E-cadherin and β-catenin. Providing insights into cell adhesion and signaling pathways, crucial for understanding tissue organization and the progression of various diseases, including carcinoid tumors.
Staining of E-cadherin/β-catenin interaction in TMA, (A) Normal colon, (B) Signet-ring cell carcinoma, (C) Carcinoid tumor (D) Adrenal gland (negative control) using NaveniBright BOND RX HRP.
Staining of E-cadherin/β-catenin interaction in TMA, (A) Normal colon, (B) Normal colon, (C) Colon cancer using NaveniFlex BOND RX Atto647N
Understanding Protein Function and Signaling Pathways
With Naveni® kits for BOND RX/RXm, researchers can investigate protein function and signaling events within the tumor microenvironment, shedding light on key biological processes and uncovering new avenues for therapeutic intervention. An example is the dynamic interplay between E-cadherin and β-catenin. Providing insights into cell adhesion and signaling pathways, crucial for understanding tissue organization and the progression of various diseases, including carcinoid tumors.
Staining of E-cadherin/β-catenin interaction in TMA, (A) Normal colon, (B) Signet-ring cell carcinoma, (C) Carcinoid tumor (D) Adrenal gland (negative control) using NaveniBright™ BOND RX HRP
Staining of E-cadherin/β-catenin interaction in TMA, (A) Normal colon, (B) Normal colon, (C) Colon cancer using NaveniFlex BOND RX Atto647N
Benefits of Naveni® kits for BOND RX/RXm
- Enhanced Research Insights: Clear visualization of protein interactions and post-translational modifications in tissue microenvironments
- Improved Efficiency and Reproducibility: Automated assay workflows reduce hands-on time and minimize variability
- Superior Productivity: Time-saving automation and streamlined workflows maximize research productivity enabling high throughput studies using proximity ligation assay.
- FFPE optimized for 30 tissue slides
- Validated on FFPE tissue and cell samples
- Pre-defined protocols in BOND RX software
Benefits of Naveni® kits for BOND RX/RXm
- Enhanced Research Insights: Clear visualization of protein interactions and post-translational modifications in tissue microenvironments
- Improved Efficiency and Reproducibility: Automated assay workflows reduce hands-on time and minimize variability
- Superior Productivity: Time-saving automation and streamlined workflows maximize research productivity enabling high throughput studies using proximity ligation assay.
- FFPE optimized for 30 tissue slides
- Validated on FFPE tissue and cell samples
- Pre-defined protocols in BOND RX software
Extensive validation
The kits have undergone thorough validation.
NaveniBright BOND RX HRP and NaveniFlex BOND RX Atto647N/Red
NaveniBright BOND RX HRP and NaveniFlex BOND RX Atto647N/Red have been validated on a diverse range of FFPE tissues and five different interaction assays (PD1/PD-L1, CD8/MHC-I, Mesothelin/Mucin, E-Cadherin/Beta-Catenin and Zap70/Lat) and dual recognition assay (HER2/HER2).
Naveni® PD1/PD-L1 BOND RX HRP and Naveni® PD1/PD-L1 BOND RX Atto647N/Red
Naveni® PD1/PD-L1 BOND RX HRP and Naveni® PD1/PD-L1 BOND RX Atto647N/Red have been validated on a diverse range of FFPE tissues, lung cancer, ovary cancer, squamous cell carcinomas from head and neck cancers, lymph nodes, and tonsils. Adrenal glands or PDL1 positive cell lines were utilized as biological negative control.
The validation protocol entailed a thorough comparative analysis between the automated kits and the NaveniBright HRP, NaveniFlex BOND RX Atto647N/Red, Naveni® PD1/PD-L1 HRP and Naveni® PD1/PD-L1 BOND RX Atto647N/Red, employing the manual methodology. Noteworthy, the kits were implemented at three distinct research sites, each utilizing their respective BOND RX/RXm systems, ensuring the establishment of robust performance characteristics across varied experimental settings.
Automated method gives the same outcome as manual method, read application note for more comparisons.
Extensive validation
The kits have undergone thorough validation.
NaveniBright BOND RX HRP and NaveniFlex BOND RX Atto647N/Red
NaveniBright BOND RX HRP and NaveniFlex BOND RX Atto647N/Red have been validated on a diverse range of FFPE tissues and five different interaction assays (PD1/PD-L1, CD8/MHC-I, Mesothelin/Mucin, E-Cadherin/Beta-Catenin and Zap70/Lat) and dual recognition assay (HER2/HER2).
Naveni® PD1/PD-L1 BOND RX HRP and Naveni® PD1/PD-L1 BOND RX Atto647N/Red
Naveni® PD1/PD-L1 BOND RX HRP and Naveni® PD1/PD-L1 BOND RX Atto647N/Red have been validated on a diverse range of FFPE tissues, lung cancer, ovary cancer, squamous cell carcinomas from head and neck cancers, lymph nodes, and tonsils. Adrenal glands or PDL1 positive cell lines were utilized as biological negative control.
The validation protocol entailed a thorough comparative analysis between the automated kits and the NaveniBright HRP, NaveniFlex BOND RX Atto647N/Red, Naveni® PD1/PD-L1 HRP and Naveni® PD1/PD-L1 BOND RX Atto647N/Red, employing the manual methodology. Noteworthy, the kits were implemented at three distinct research sites, each utilizing their respective BOND RX/RXm systems, ensuring the establishment of robust performance characteristics across varied experimental settings.
Automated method gives the same outcome as manual method, read application note for more comparisons.
Read more
Download the application note High-throughput fully automated in situ proximity ligation assays for spatial interactomics (PDF) for detailed information about the validation and beta test results.
Naveni® kits for BOND RX/RXm
NaveniBright BOND RX HRP
NaveniBright BOND RX HRP is offering flexibility tailored to your unique primary antibodies and targets. Use your own primary antibodies, validated for IHC on BOND RX/RXm, and add NaveniBright BOND RX HRP for proximity ligation assay and illuminate the function of your protein targets.
Naveni® PD1/PD-L1 BOND RX HRP
Naveni® PD1/PD-L1 BOND RX HRP is visualizing the precise interaction of PD1/PD-L1 within the tissue microenvironment for detailed spatial insights. Primary antibodies are optimized and included in the kit.
NaveniFlex BOND RX Atto647N/Red
NaveniFlex BOND RX Atto647N/Red is offering flexibility tailored to your unique primary antibodies and targets. Use your own primary antibodies, validated for IHC on BOND RX/RXm, and add NaveniFlex BOND RX Atto647N/Red for proximity ligation assay and illuminate the function of your protein targets.
Naveni® PD1/PD-L1 BOND RX Atto647N/Red
Naveni® PD1/PD-L1 BOND RX Atto647N/Red is visualizing the precise interaction of PD1/PD-L1 within the tissue microenvironment for detailed spatial insights. Primary antibodies are optimized and included in the kit.
Naveni® kits for BOND RX/RXm
NaveniBright BOND RX HRP
NaveniBright BOND RX HRP is offering flexibility tailored to your unique primary antibodies and targets. Use your own primary antibodies, validated for IHC on BOND RX/RXm, and add NaveniBright BOND RX HRP for proximity ligation assay and illuminate the function of your protein targets.
Naveni® PD1/PD-L1 BOND RX HRP
Naveni® PD1/PD-L1 BOND RX HRP is visualizing the precise interaction of PD1/PD-L1 within the tissue microenvironment for detailed spatial insights. Primary antibodies are optimized and included in the kit.
NaveniFlex BOND RX Atto647N/Red
NaveniFlex BOND RX Atto647N/Red is offering flexibility tailored to your unique primary antibodies and targets. Use your own primary antibodies, validated for IHC on BOND RX/RXm, and add NaveniFlex BOND RX Atto647N/Red for proximity ligation assay and illuminate the function of your protein targets.
Naveni® PD1/PD-L1 BOND RX Atto647N/Red
Naveni® PD1/PD-L1 BOND RX Atto647N/Red is visualizing the precise interaction of PD1/PD-L1 within the tissue microenvironment for detailed spatial insights. Primary antibodies are optimized and included in the kit.
In partnership with Leica Biosystems.