Fully automated in situ proximity ligation assays

Naveni® kits for BOND RX/RXm

Automated detection of protein-protein interactions and post-translational modifications in situ

For the first time is proximity ligation assay available with an automated workflow. The NaveniBright™ and Naveni® PD1/PD-L1 BOND RX HRP introduces two novel products seamlessly integrating chromogenic readout automation on the BOND RX/RXm Fully Automated Research Stainer. These in situ kits are meticulously crafted based on the cutting-edge Naveni® in situ proximity ligation technology.

Automated detection of protein-protein interactions and post-translational modifications in situ

For the first time is proximity ligation assay available with an automated workflow. The NaveniBright™ and Naveni® PD1/PD-L1 BOND RX HRP introduces two novel products seamlessly integrating chromogenic readout automation on the BOND RX/RXm Fully Automated Research Stainer. These in situ kits are meticulously crafted based on the cutting-edge Naveni® in situ proximity ligation technology.

Leica BOND RX/RXm and Naveni Workflow
  • Detect protein-protein interactions and post-translational modifications efficiently using the BOND RX/RXm, ensuring high precision and accuracy.
  • Visualize specific protein targets like PD1/PD-L1 while preserving intact cell and tissue morphology, providing a holistic understanding of spatial protein relationships.
  • Enhance your research efficiency and reproducibility through a seamlessly integrated automated workflow on BOND RX/RXm, optimizing your investigative process and achieving high throughput.
  • Save valuable time with the automated workflow, reducing hands-on time and maximizing overall research productivity.
  • FFPE optimized, reagents sufficient for 30 FFPE tissue slides.
Leica BOND RX/RXm and Naveni Workflow
  • Detect protein-protein interactions and post-translational modifications efficiently using the BOND RX/RXm, ensuring high precision and accuracy.
  • Visualize specific protein targets like PD1/PD-L1 while preserving intact cell and tissue morphology, providing a holistic understanding of spatial protein relationships.
  • Enhance your research efficiency and reproducibility through a seamlessly integrated automated workflow on the Leica BOND RX/RXm, optimizing your investigative process and achieving high throughput.
  • Save valuable time with the automated workflow, reducing hands-on time and maximizing overall research productivity.
  • FFPE optimized, reagents sufficient for 30 FFPE tissue slides.

Naveni® kits for BOND RX/RXm

NaveniBright™ BOND RX HRP

NaveniBright™ BOND RX HRP is offering flexibility tailored to your unique primary antibodies and targets. Use your own primary antibodies, validated for IHC on BOND RX/RXm, and add NaveniBright BOND RX HRP for proximity ligation assay and illuminate the function of your protein targets.

Naveni® PD1/PD-L1 BOND RX HRP

Naveni® PD1/PD-L1 BOND RX HRP is visualizing the precise interaction of PD1/PD-L1 within the tissue microenvironment for detailed spatial insights. Primary antibodies are optimized and included in the kit.

Naveni® kits for BOND RX/RXm

NaveniBright™ BOND RX HRP

NaveniBright™ BOND RX HRP is offering flexibility tailored to your unique primary antibodies and targets. Use your own primary antibodies, validated for IHC on BOND RX/RXm, and add NaveniBright BOND RX HRP for proximity ligation assay and illuminate the function of your protein targets.

Naveni® PD1/PD-L1 BOND RX HRP

Naveni® PD1/PD-L1 BOND RX HRP is visualizing the precise interaction of PD1/PD-L1 within the tissue microenvironment for detailed spatial insights. Primary antibodies are optimized and included in the kit.

Precise Visualization of PD1/PD-L1 Interactions using Naveni® PD1/PD-L1 BOND RX

The Naveni® PD1/PD-L1 BOND RX HRP offer a comprehensive solution for studying PD1/PD-L1 interactions within the tissue microenvironment. With precise visualization capabilities, researchers can gain deeper insights into the dynamics of immune checkpoint pathways, facilitating the development of targeted immunotherapies for cancer treatment.

Naveni® PD1/PD-L1 BOND RX HRP was used to detect PD1/PD-L1 interaction in head neck cancer (A, B), Adrenal gland was used as neg biological control (C).

Precise Visualization of PD1/PD-L1 Interactions using Naveni® PD1/PD-L1 BOND RX

The Naveni® PD1/PD-L1 BOND RX HRP offer a comprehensive solution for studying PD1/PD-L1 interactions within the tissue microenvironment. With precise visualization capabilities, researchers can gain deeper insights into the dynamics of immune checkpoint pathways, facilitating the development of targeted immunotherapies for cancer treatment.

Naveni® PD1/PD-L1 BOND RX HRP was used to detect PD1/PD-L1 interaction in head neck cancer (A, B), Adrenal gland was used as neg biological control (C).

Investigating Protein-Protein Interactions

Naveni® kits for BOND RX/RXm Fully Automated Research Stainer enable researchers to explore complex protein-protein interactions with high precision and accuracy using their own primary antibodies. By visualizing these interactions within the spatial context of cells and tissues, researchers can unravel intricate signaling networks and uncover novel therapeutic targets for various diseases.

An example is the interactions between CD8 and MHC-I. Understanding these interactions is vital for elucidating the role of cytotoxic T cells in immune surveillance and their involvement in diseases such as cancer and autoimmune disorders.

CD8 MHCI interaction in breast cancer NaveniBright BOND RX HRP

Staining of CD8/MHCI interaction in breast cancer using NaveniBright BOND RX HRP (A, B positive signals, C negative control)

Investigating Protein-Protein Interactions

Naveni® kits for BOND RX/RXm Fully Automated Research Stainer enable researchers to explore complex protein-protein interactions with high precision and accuracy using their own primary antibodies. By visualizing these interactions within the spatial context of cells and tissues, researchers can unravel intricate signaling networks and uncover novel therapeutic targets for various diseases.

An example is the interactions between CD8 and MHC-I. Understanding these interactions is vital for elucidating the role of cytotoxic T cells in immune surveillance and their involvement in diseases such as cancer and autoimmune disorders.

Staining of CD8/MHCI interaction in breast cancer using NaveniBright BOND RX HRP (A, B positive signals, C negative control)

Understanding Protein Function and Signaling Pathways

With Naveni® kits for BOND RX/RXm, researchers can investigate protein function and signaling events within the tissue microenvironment, shedding light on key biological processes and uncovering new avenues for therapeutic intervention. An example is the dynamic interplay between E-Cadherin and Beta-catenin. Providing insights into cell adhesion and signaling pathways, crucial for understanding tissue organization and the progression of various diseases, including carcinoid tumors.

Ecadherin Beta catenin interaction TMA NaveniBright BOND RX HRP-web
Ecadherin Beta catenin interaction TMA NaveniBright BOND RX HRP-web
Ecadherin Beta catenin interaction TMA NaveniBright BOND RX HRP-web
Ecadherin Beta catenin interaction TMA NaveniBright BOND RX HRP-web

Staining of E cadherin/Beta catenin interaction in TMA, A) Normal colon, B) Signet-ring cell carcinoma, C) Carcinoid tumor D) Adrenal gland (neg ctrl) using NaveniBright BOND RX HRP

Understanding Protein Function and Signaling Pathways

With Naveni® kits for BOND RX/RXm, researchers can investigate protein function and signaling events within the tissue microenvironment, shedding light on key biological processes and uncovering new avenues for therapeutic intervention. An example is the dynamic interplay between E-Cadherin and Beta-catenin. Providing insights into cell adhesion and signaling pathways, crucial for understanding tissue organization and the progression of various diseases, including carcinoid tumors.

Staining of E cadherin/Beta catenin interaction in TMA, A) Normal colon, B) Signet-ring cell carcinoma, C) Carcinoid tumor D) Adrenal gland (neg ctrl) using NaveniBright BOND RX HRP

Benefits of Naveni® kits for BOND RX/RXm

  • Enhanced Research Insights: Clear visualization of protein interactions and post-translational modifications in tissue microenvironments
  • Improved Efficiency and Reproducibility: Automated assay workflows reduce hands-on time and minimize variability
  • Superior Productivity: Time-saving automation and streamlined workflows maximize research productivity enabling high throughput studies using proximity ligation assay.
  • FFPE optimized for 30 tissue slides
  • Validated on FFPE tissue and cell samples
  • Standard Template in Leica BOND software
Naveni for Bond Workflow Benefits

Benefits of Naveni® kits for BOND RX/RXm

  • Enhanced Research Insights: Clear visualization of protein interactions and post-translational modifications in tissue microenvironments
  • Improved Efficiency and Reproducibility: Automated assay workflows reduce hands-on time and minimize variability
  • Superior Productivity: Time-saving automation and streamlined workflows maximize research productivity enabling high throughput studies using proximity ligation assay.
  • FFPE optimized for 30 tissue slides
  • Validated on FFPE tissue and cell samples
  • Standard Template in Leica BOND software
Naveni for Bond Workflow Benefits

Extensive validation

The kits has undergone thorough validation.

NaveniBright BOND RX HRP

NaveniBright BOND RX HRP has been validated on a diverse range of FFPE tissues and five different interaction assays (PD1/PD-L1, CD8/MHC-I, Mesothelin/Mucin, E-Cadherin/Beta-Catenin and Zap70/Lat) and dual recognition assay (HER2/HER2).

Naveni® PD1/PD-L1 BOND RX HRP

Naveni® PD1/PD-L1 BOND RX HRP has been validated on a diverse range of FFPE tissues, lung cancer, ovary cancer, squamous cell carcinomas from head and neck cancers, lymph nodes, and tonsils. Adrenal glands or PDL1 positive cell lines were utilized as biological negative control.

The validation protocol entailed a thorough comparative analysis between the automated kits and the NaveniBright HRP and Naveni® PD1/PD-L1 HRP, employing the manual methodology. Noteworthy, the kits were implemented at three distinct research sites, each utilizing their respective BOND RX/RXm systems, ensuring the establishment of robust performance characteristics across varied experimental settings.

Automated method gives the same outcome as manual method

Automated method gives the same outcome as manual method, read application note for more comparisons.

Extensive validation

The kits has undergone thorough validation.

NaveniBright BOND RX HRP

NaveniBright BOND RX HRP has been validated on a diverse range of FFPE tissues and five different interaction assays (PD1/PD-L1, CD8/MHC-I, Mesothelin/Mucin, E-Cadherin/Beta-Catenin and Zap70/Lat) and dual recognition assay (HER2/HER2).

Naveni® PD1/PD-L1 BOND RX HRP

Naveni® PD1/PD-L1 BOND RX HRP has been validated on a diverse range of FFPE tissues, lung cancer, ovary cancer, squamous cell carcinomas from head and neck cancers, lymph nodes, and tonsils. Adrenal glands or PDL1 positive cell lines were utilized as biological negative control.

The validation protocol entailed a thorough comparative analysis between the automated kits and the NaveniBright HRP and Naveni® PD1/PD-L1 HRP, employing the manual methodology. Noteworthy, the kits were implemented at three distinct research sites, each utilizing their respective BOND RX/RXm systems, ensuring the establishment of robust performance characteristics across varied experimental settings.

Automated method gives the same outcome as manual method, read application note for more comparisons.

Read more

Download the application note High-throughput fully automated in situ proximity ligation assays for spatial interactomics (PDF) for detailed information about the validation and beta test results.

Visit our FAQ section.

Read more

Download the application note High-throughput fully automated in situ proximity ligation assays for spatial interactomics (PDF) for detailed information about the validation and beta test results.

Visit our FAQ section.

In partnership with Leica Biosystems.

In partnership with Leica Biosystems.