Introducing Naveni® Plex
Naveni® Plex enables simultaneous detection of 3 separate targets or protein-protein interactions with high specificity, sensitivity and resolution. It’s a ready-to-use kit with seamless integration with standard IF workflows.
The next generation in situ proximity ligation assay
Introducing Naveni® Plex
Naveni® Plex enables simultaneous detection of 3 separate targets or protein-protein interactions with high specificity, sensitivity and resolution. It’s a ready-to-use kit with seamless integration with standard IF workflows.
Uncover complex protein interactions in the tumor microenvironment with Naveni® Plex, the groundbreaking isPLA multiplex solution designed to simultaneously detect:
- PD1/PD-L1 interactions – illuminating activated immune checkpoint pathways
- CD8/MHC-I interactions – revealing initiation of critical T-cell activation processes
- CD3 presence – tracking a key T-cell marker
Understand immune evasion and mechanisms
Estimate the relative levels of T-cell infiltration (CD3 and CD8), cytotoxic engagement (CD8/MHC-I), and immune inhibition (PD1/PD-L1) to investigate how immune evasion correlates with T-cell activation and gain insights into tumors blocking immune attacks.
Workflow
Step 1
Navenibodies bind to their target proteins
Step 2
Proximally bound Navenibodies connect through a circularized DNA ”bridge”
Step 3
The connector DNA is amplified and detected by multiple fluorescently labeled oligos
Multiplex isPLA providing functional proteomics
Mapping multiple protein interactions simultaneously in the cancer immune interface, going beyond expression to reveal functionality.
Optimized and ready to use
Curated and validated protein targets for improved understanding of the immune landscape in tissue.
Enhanced sensitivity & specificity
Dual antibody recognition with signal amplification ensures precise detection of even the most challenging targets in a multiplex setting.
Naveni® Plex kit
Naveni® Plex is the first multiplex in situ proximity ligation assay, with a fluorescent readout, enabling the simultaneous detection of 3 separate targets, protein–protein interactions and/or single protein expression, with high specificity and sensitivity. The kit has been validated on FFPE human tissue samples.