Description
The NaveniFlex™ BOND RX Atto647N is an in situ proximity ligation assay kit using BOND RX/RXm Fully Automated Research Stainer. The kit has been validated on FFPE human tissue samples. For research use only. Not for use in diagnostic procedures
The kit is based on the Naveni® in situ proximity ligation technology with automated workflow. It uses two Navenibodies linked to proprietary oligo arms. The two Navenibodies included in the kit will bind primary antibodies of mouse and rabbit origin. When the Navenibodies are in close proximity, a strong and distinct signal is generated. Read more about the automated technology here.
Product code: 60009
Included in the NaveniFlex BOND RX Atto647N kit:
- Anti-mouse Navenibody
- Anti-rabbit Navenibody
- Buffers for blocking and dilutions and detection reagents
- Reagents sufficient for 30 FFPE tissue slides, including dead volumes
Required Materials and Reagents to run NaveniFlex BOND RX Atto647N:
These materials are not included in the kit and must be purchased separately.
| Antibodies | Description |
|---|---|
| Mouse Antibody 1 | Use an antibody of choice or a Naveni target specific kit. |
| Rabbit Antibody 2 | Use an antibody of choice or a Naveni target specific kit. |
| Leica, Cat # | Description |
|---|---|
| OPT9049 | BOND titration kit (6ml) |
| DS9455
(DS9777) |
BOND Research Detection System
(BOND Research Detection System 2) |
| S21.4611 | BOND Universal Covertiles |
| S21.0304 | Slide Tray |
| S21.1003 | Reagent Tray |
| S21.4564 | Slide Labels and Printer Ribbon |
| AR9222 | BOND Dewax Solution |
| AR9961 | BOND Epitope Retrieval Solution 1 |
| AR9640 | BOND Epitope Retrieval Solution 2 |
| AR9590 | BOND Wash Solution 10X Concentrate |
| CS9100 | BOND Aspirating Probe Cleaning Kit |
| Laboratory Materials | Vendor, Cat# |
|---|---|
| Mounting medium for fluorescent dyes | Sigma-Aldrich, F6182* |
| Coverslip for microscope slides | |
| DAPI (diluted in PBS to 1 µg/mL)** | Thermo Fisher Scientific, 62248 |
| Deionized water | |
| Reagent Grade Alcohol | Refer to the BOND RX 7.0 User Manual |
| TBS-T (T=Tween) |
*Fluoroshield mounting medium or any mounting medium compatible with fluorescent dyes.
**It is possible to exclude the DAPI staining step in the instrument stain with DAPI off-board.
Software requirements to run NaveniFlex BOND RX
- BOND RX v7.0 software
- BXD 40
Documents
-
- Product sheet NaveniFlex BOND RX Atto647N (PDF)
- Instructions for use NaveniFlex BOND RX Atto647N (PDF)
- SDS (PDF)
- Leica Biosystems BOND RX Brochure (PDF)
- FAQ