NaveniFlex BOND RX Red

29.500 kr

Detect proteins, protein – protein interactions, or protein modifications with Naveni® in situ technology using BOND RX/RXm Fully Automated Research Stainer. The kit should be used with the user’s primary antibodies of choice (raised in mouse and rabbit). Detection with Texas Red fluorophore is included.

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Category: SKU: 60010

Description

The NaveniFlex™ BOND RX Red is an in situ proximity ligation assay kit using BOND RX/RXm Fully Automated Research Stainer. The kit has been validated on FFPE human tissue samples. For research use only. Not for use in diagnostic procedures

The kit is based on the Naveni® in situ proximity ligation technology with automated workflow. It uses two Navenibodies linked to proprietary oligo arms. The two Navenibodies included in the kit will bind primary antibodies of mouse and rabbit origin. When the Navenibodies are in close proximity, a strong and distinct signal is generated. Read more about the automated technology here.

Product code: 60010

 

Included in the NaveniFlex BOND RX Red kit:

  • Anti-mouse Navenibody
  • Anti-rabbit Navenibody
  • Buffers for blocking and dilutions and detection reagents
  • Reagents sufficient for 30 FFPE tissue slides, including dead volumes

 

Required Materials and Reagents to run NaveniFlex BOND RX Red:

These materials are not included in the kit and must be purchased separately.

Antibodies Description
Mouse Antibody 1 Use an antibody of choice or a Naveni target specific kit.
Rabbit Antibody 2 Use an antibody of choice or a Naveni target specific kit.

 

Leica, Cat # Description
OPT9049 BOND titration kit (6ml)
DS9455

(DS9777)

BOND Research Detection System

(BOND Research Detection System 2)

S21.4611 BOND Universal Covertiles
S21.0304 Slide Tray
S21.1003 Reagent Tray
S21.4564 Slide Labels and Printer Ribbon
AR9222 BOND Dewax Solution
AR9961 BOND Epitope Retrieval Solution 1
AR9640 BOND Epitope Retrieval Solution 2
AR9590 BOND Wash Solution 10X Concentrate
CS9100 BOND Aspirating Probe Cleaning Kit

 

Laboratory Materials Vendor, Cat#
Mounting medium for fluorescent dyes Sigma-Aldrich, F6182*
Coverslip for microscope slides
DAPI (diluted in PBS to 1 µg/mL)** Thermo Fisher Scientific, 62248
Deionized water
Reagent Grade Alcohol Refer to the BOND RX 7.0 User Manual
TBS-T (T=Tween)

*Fluoroshield mounting medium or any mounting medium compatible with fluorescent dyes.
**It is possible to exclude the DAPI staining step in the instrument stain with DAPI off-board.

 

Software requirements to run NaveniFlex BOND RX

  • BOND RX v7.0 software
  • BXD 40

 

Documents

Additional information

Target

Flexible

Samples type

Cells, Tissue

Reactivity

Mouse & Rabbit

Readout

Fluorescence

Method

Automated with BOND RX/RXm

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